Build Status

Leonardo#

A toolset to remove sample-induced aberrations in light sheet microscopy images

Leonardo is an AI-empowered image processing toolbox that is able to resolve sample-induced aberrations for light-sheet fluorescence microscopy: (1) Leonardo-DeStripe removes the stripe artifacts caused by light absorption; (2) Leonardo-Fuse reconstructs one single high-quality image from dual-sided illumination and/or dual-sided detection while eliminating optical distortions (ghosts) caused by light refraction.

leonardo title figure

Manuscript#

Preprint is available here.

Installation

How to install Leonardo.

Tutorials

Get started with example notebooks and video tutorials.

Tuning Guidance

Parameter tuning and validation workflow.

API Reference

Detailed descriptions of Leonardo modules: DeStripe, Fuse_illu, Fuse_det.